Translational_Unit

Part:BBa_K759006:Design

Designed by: Ami Kawahata   Group: iGEM12_HokkaidoU_Japan   (2012-09-19)


B0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 139
    Illegal NgoMIV site found at 235
    Illegal NgoMIV site found at 243
    Illegal NgoMIV site found at 255
    Illegal NgoMIV site found at 352
    Illegal NgoMIV site found at 592
    Illegal NgoMIV site found at 733
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nothing to remark.


Source

PhaA was cloned from pGEM phbCAB which carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.


References